polyclonal rabbit anti human hoxc6 antibody  (Bioss)

Journal: Translational Oncology

Article Title: Comprehensive analysis and experimental verification of the mechanism of action of T cell-mediated tumor-killing related genes in Colon adenocarcinoma

doi: 10.1016/j.tranon.2024.101918

Figure Lengend Snippet: The target sequences for NPM1 siRNA.

Article Snippet: The tissue sections through deparaffinization and dehydration were incubated with polyclonal rabbit anti-human HOXC6 antibodies (1:500, Abcam, ab151575) overnight at 4 °C after epitope retrieval, H2O2 treatment, and non-specific antigens blocking.

Techniques: Sequencing

Journal: Translational Oncology

Article Title: Comprehensive analysis and experimental verification of the mechanism of action of T cell-mediated tumor-killing related genes in Colon adenocarcinoma

doi: 10.1016/j.tranon.2024.101918

Figure Lengend Snippet: A list of the sequences of primer pairs for target genes.

Article Snippet: The tissue sections through deparaffinization and dehydration were incubated with polyclonal rabbit anti-human HOXC6 antibodies (1:500, Abcam, ab151575) overnight at 4 °C after epitope retrieval, H2O2 treatment, and non-specific antigens blocking.

Techniques: Sequencing

Journal: Translational Oncology

Article Title: Comprehensive analysis and experimental verification of the mechanism of action of T cell-mediated tumor-killing related genes in Colon adenocarcinoma

doi: 10.1016/j.tranon.2024.101918

Figure Lengend Snippet: HOXC6 is elevated in colon cancer tissues. (A-E) The expression of C11orf96, CXCL9, HOXC6, VISG4 and CXCL13 in NCM460, SW480 and SW620 was detected by qRT-PCR and analyzed for relative quantification. (F-G) The expression of HOXC6 in cancer and paracancer tissues was detected and quantified using IHC. (H) The inhibition efficiency of small interfering RNAs was detected and analyzed for relative quantification in SW480 cell line. (I) Cell viability of SW480 was assayed after inhibition of HOXC6 expression in SW480 cell line. (J) The inhibition efficiency of small interfering RNAs was detected and analyzed for relative quantification in SW620 cell line. (K) Cell viability of SW480 was assayed after inhibition of HOXC6 expression in SW620 cell line. N = 3. *≤0.05, **≤0.01, ***≤0.001, ****≤0.0001. N = 6/3, The results are presented as mean ± SD.

Article Snippet: The tissue sections through deparaffinization and dehydration were incubated with polyclonal rabbit anti-human HOXC6 antibodies (1:500, Abcam, ab151575) overnight at 4 °C after epitope retrieval, H2O2 treatment, and non-specific antigens blocking.

Techniques: Expressing, Quantitative RT-PCR, Inhibition

Journal: Translational Oncology

Article Title: Comprehensive analysis and experimental verification of the mechanism of action of T cell-mediated tumor-killing related genes in Colon adenocarcinoma

doi: 10.1016/j.tranon.2024.101918

Figure Lengend Snippet: HOXC6 promotes invasion and immunosuppression of colon cancer cell lines. (A-B) Alterations in cell migration as well as invasive capacity after inhibition of HOXC6 expression in SW480 and SW620 cell lines. (C-G) The expression levels of CXCL1, CXCL8, IL1β, IL4 and IL10 in SW480 cell line were detected by qRT-PCR before and after HOXC6 inhibition. (H-L) The expression levels of CXCL1, CXCL8, IL1β, IL4 and IL10 in SW620 cell line were detected by qRT-PCR before and after HOXC6 inhibition. N = 3. *≤0.05, **≤0.01, ***≤0.001, ****≤0.0001. N = 6/3, The results are presented as mean ± SD.

Article Snippet: The tissue sections through deparaffinization and dehydration were incubated with polyclonal rabbit anti-human HOXC6 antibodies (1:500, Abcam, ab151575) overnight at 4 °C after epitope retrieval, H2O2 treatment, and non-specific antigens blocking.

Techniques: Migration, Inhibition, Expressing, Quantitative RT-PCR